Product features

This product, 2×SYBR green qPCR MIX, comes in a single tube containing all the components required for PCR amplification and detection, including Taq DNA poLymerase, SYBR green I dye, High ROX Reference Dye, dNTPs, Mg2+, and PCR buffer.
SYBR green I dye is a green fluorescent dye that binds to the double-stranded DNA (double-strand DNA, dsDNA) double helix minor groove region.SYBR green I fluoresces weakly in the free state, but once it binds to double-stranded DNA, its fluorescence is greatly enhanced. This makes it possible to quantify the amount of double-stranded DNA produced during PCR amplification by detecting the fluorescence intensity.
ROX is used as a correction dye to correct for fluorescence fluctuations unrelated to PCR, thus minimizing spatial differences. Such differences can be caused by a variety of factors, such as pipette error or sample evaporation. Different fluorescence quantification instruments have different needs for ROX, and this product is suitable for fluorescence quantification analyzers that require High ROX correction.